In Vitro Genetic Recombination of Bacteriophage
نویسندگان
چکیده
منابع مشابه
Genetic recombination in bacteriophage lambda
© The Author 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Genetic recombination in bacteriop...
متن کاملGenetic recombination of bacteriophage T7 DNA in vitro. IV. Asymmetry of recombination frequencies caused by polarity of DNA packaging.
We have demonstrated recombination of bacteriophage T7 DNA in vitro. An extract of Escherichia coli B cells infected with wild-type T7(T7+) is incubated with mature DNA extracted from T7 phage. Packaging of the exogenous DNA within the phage head appears to be preceded by recombination of exogenously added DNA with DNA present in the extracts. In order to detect the recombination, we used an ex...
متن کاملIntegrative recombination of bacteriophage lambda DNA in vitro.
An in vitro system for the production of integrative recombinant DNA of bacteriophage lambda is described. The in vitro recombination mimics the in vivo integration of viral DNA into host DNA in its requirement for int gene product, for the presence of a thermolabile component, and for the limitation of the recombination to a pair of specialized sites (attachment sites) on the DNA. The enzymes ...
متن کاملGenetic recombination of bacteriophage A DNAs in Xenopus oocytes
Pairs of genetically marked bacteriophage A DNAs have been injected into Xenopus laevis oocyte nuclei. After suitable incubation, DNA was recovered and packaged into phage particles in vitro. When these were plated onto a selective host, phage recombinant for parental markers were observed. Recombination was dependent on both parents being present in the same oocyte nucleus and was roughly prop...
متن کاملBacteriophage T4 DNA topoisomerase mediates illegitimate recombination in vitro.
We have found that purified T4 DNA topoisomerase promotes recombination between two phage lambda DNA molecules in an in vitro system. In this cross, T4 DNA topoisomerase alone is able to catalyze the recombination and produce a linear monomer recombinant DNA that can be packaged in vitro. ATP is not required for this recombination, while oxolinic acid stimulates it. The recombinant DNA molecule...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.6.2496